Coding

Part:BBa_K3463002:Design

Designed by: Elouen Le Garrec   Group: iGEM20_Grenoble_Alpes   (2020-10-22)


algL-Alginate Lyase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 173
    Illegal NgoMIV site found at 973
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1140
    Illegal BsaI.rc site found at 298


Design Notes

AlgL is regulated by AlgB in Pseudomonas aeruginosa genome. Should be added if production in E. coli is insufficient. Biosynthesis is higher when in presence of high salt concentrations. Characterization of the Pseudomonas aeruginosa alginate lyase gene (algL): cloning, sequencing, and expression in Escherichia coli.N L Schiller, S R Monday, C M Boyd, N T Keen, D E Ohman Journal of Bacteriology Aug 1993, 175 (15) 4780-4789; DOI:10.1128/jb.175.15.4780-4789.1993 Compatible RFC10



Source

Genomic extraction by PCR on Pseudomonas aeruginosa PAO1. Blanco-Cabra, N., Paetzold, B., Ferrar, T. et al. Characterization of different alginate lyases for dissolving Pseudomonas aeruginosa biofilms. Sci Rep 10, 9390 (2020).


References